Single-Molecule Real-time (SMRT) sequencing Market

The single-molecule real-time (SMRT) sequencing market is expected to reach USD 5.32 billion by 2030, up from USD 2.92 billion in 2025, with a significant CAGR of 12.8% between 2025 and 2030. Several key factors, including the demand for long reads with high accuracy and improved visibility of structural variants and complex genomic regions, drive the growth of the single-molecule real-time (SMRT) sequencing market. Several key players in the SMRT Sequencing industry include Pac Bio (US), Eurofins Scientific (Luxembourg), Illumina, Inc. (US), Thermo Fisher, Inc.  (US), Agilent Technologies (US), BGI Group (China), among others. Companies in this space are adopting expansion strategies to strengthen their global footprint and advance their technology portfolios. In June 2025, PacBio and Ultima Genomics entered a strategic collaboration to develop high-throughput solutions that combine SMRT long-read data with cost-efficient short-read sequencing for large populations and clinical studies.

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Illumina, Inc. (US) is one of the most influential players in the single-molecule real-time (SMRT) sequencing market by providing short-read platforms that many labs pair with long-read SMRT data for hybrid assemblies and population-scale studies. Through its NovaSeq and NextSeq systems, library preparation kits, and cloud informatics, the company supports large human genome, oncology, and screening programs that increasingly incorporate long-read confirmation and phasing for challenging regions. Illumina’s investment in linked-read-like approaches, single-cell and spatial solutions, and multimodal data analysis strengthens its role as a key ecosystem partner rather than a direct SMRT competitor, helping customers design balanced workflows that combine depth, accuracy, and cost efficiency.

Thermo Fisher Scientific, Inc. (US) plays a central enabling role in the SMRT sequencing landscape through its Ion Torrent and capillary electrophoresis platforms, extensive library prep chemistries, and proteomics and mass spec tools used in downstream multi-omics studies. The company also supplies sample-prep automation, QC instruments, and informatics solutions that help labs standardize long-read workflows, including those built around PacBio SMRT systems. With a broad footprint in clinical and translational labs, Thermo Fisher is well-positioned to support the move toward integrated DNA–RNA–protein analysis, in which SMRT sequencing provides high-fidelity, long-read genomes. At the same time, Thermo’s tools handle complementary assays and routine diagnostics.

Agilent Technologies, Inc. (US) contributes to the SMRT sequencing market primarily as an upstream and QC partner, offering high-precision LC, GC, electrophoresis, and Bioanalyzer/Fragment Analyzer platforms widely used for SMRT library quality control and sizing. Its target-enrichment kits, automation solutions, and analytical instruments help researchers optimize sample quality and focus on difficult genomic regions before running long-read SMRT experiments. By focusing on robust front-end prep, method development, and data validation, Agilent enables smoother adoption of SMRT workflows in research and clinical labs that require reliable, audit-ready results.

Market Ranking

The single-molecule real-time (SMRT) sequencing market is characterized by significant consolidation. These major companies reinforce their positions through strategic acquisitions and advanced technological capabilities. Illumina’s high-throughput short-read platforms and cloud tools are frequently paired with SMRT long reads for hybrid genome assemblies and large-scale population studies, providing customers with an efficient balance of cost and completeness. Thermo Fisher contributes library-prep chemistries, automation, QC systems, and informatics that help standardize end-to-end long-read workflows in translational and clinical labs. Agilent underpins the front end with reliable sample quality assessment, electrophoresis, and fragment analysis solutions, thereby improving library integrity and overall SMRT data quality.

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Single-molecule Real-time (SMRT) Sequencing Market by Offering (Products, Services), Workflow (Sequencing, Data Analysis), Application (Drug Discovery, Diagnostic, Agriculture), End User (Academia, Pharma, Biotech) - Forecast to 2030

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